Catalog Number - 68140-15P

Cogent NP Amino™ is a versatile normal phase and HILIC stationary phase designed for the separation of highly polar compounds that are often difficult to retain using traditional reversed-phase methods. Manufactured on ultra-high purity silica with a propyl amino bonded phase and supplied without endcapping, this chemistry provides excellent selectivity for carbohydrates, sugars, organic acids, and other polar analytes. The stationary phase is commonly used as an alternative for USP L8 methods and is suitable for both Normal Phase and HILIC applications.

68140-15P is a UHPLC column packed with 1.8 µm NP Amino™ particles featuring a 100 Å pore size in a 4.6 mm ID x 150 mm format. The 150 mm length maximizes resolution and peak capacity for complex separations, while the sub-2 µm particle technology delivers exceptional efficiency and chromatographic performance. This column is specifically designed for high-pressure UHPLC operation and provides optimum performance when used on UHPLC systems capable of handling the elevated pressures generated by 1.8 µm particles. Typical operating flow rates are approximately 1.0–2.0 mL/min depending on mobile phase composition and system pressure limitations.

This column is particularly effective for sugars, carbohydrates, negatively charged compounds, organic acids, and compounds containing carboxylic acid functionality. Suitable for both Normal Phase and HILIC applications, it is an excellent choice when maximum resolution and enhanced retention of polar compounds are required.

Key Benefits

• NP Amino™ stationary phase

• USP L8 equivalent chemistry

• Propyl amino bonded phase

• Ultra-high purity silica support

• Suitable for Normal Phase chromatography

• Suitable for HILIC applications

• Designed for UHPLC applications

• High-efficiency 1.8 µm particles

• Exceptional peak capacity

• Maximum chromatographic resolution

Typical Applications

• UHPLC analysis

• USP L8 methods

• HILIC separations

• Normal Phase chromatography

• Carbohydrate analysis

• Sugar analysis

• Organic acid analysis

• Polar compound analysis

• Pharmaceutical analysis

• Complex sample separations

Specifications

• Product Type: UHPLC Column

• Stationary Phase: NP Amino™

• USP Classification: L8

• Chromatography Mode: Normal Phase / HILIC

• Bonded Phase: Propyl Amino

• Endcapping: Not Endcapped

• Particle Size: 1.8 µm

• Pore Size: 100 Å

• Column Dimensions: 4.6 mm ID x 150 mm

• Length: 150 mm

• Typical Flow Rate: 1.0–2.0 mL/min

• Brand: Cogent

Important Notes

• Specifically designed for UHPLC operation and high-pressure chromatography

• Delivers maximum performance when used on a UHPLC system designed for sub-2 µm particle columns

• May be used on compatible HPLC systems only if instrument pressure limits are not exceeded

• Sub-2 µm particles generate substantially higher backpressure than conventional HPLC columns

• Packed and shipped in Normal Phase solvents

• Suitable for both Normal Phase and HILIC chromatographic modes

• Excellent for sugars, carbohydrates, organic acids, and other highly polar compounds

• The 150 mm length maximizes resolution and peak capacity for complex sample separations

• Samples should be filtered through a 0.2 µm syringe filter prior to injection

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